Men will go for it if they meet 60% of the requirements. Women look for a reason "not" to go for it, so, they let that one little itty bitty thing hold them back. Don't!
So yes, take chances! That being said, if you clearly would be in WAY over your head, don't do it. An example would be an Administrative Assistant who can't get around in Word or Excel. You want to be considerate of the employer's time. If you apply for the ones that you really aren't suited for, and later, something more appropriate comes open, you don't want them looking at your resume and saying, "oh. its "her.""
If the missing skill might keep you from getting the interview, consider addressing it head-on in your cover letter. That is, AFTER you have sold yourself, add a small section to say something like "Although I have no experience in xyz, at one time I had no experience in abc. Once I was exposed to it, I was quickly able to master it, as you can see in my portfolio. I expect to be able to do the same with xyz, and in fact, have already begun studying so-and-so's work in that area."
Speaking of portfolio's, make sure yours is up to speed, and is available in whatever forums typically used by artists in your field. I know LinkedIn allows for uploading. You may want to have your own website. This way, when you apply, they can look at your work. Don't wait till the interview to make your work available!